Cube Dx' hybcell is the world's first cylindrical microarray, whereas DNA oligos are immobilised on a cylindrical coated surface. Matching DNA fragments in the sample bind to these probes on the surface. A fluorescence readout shows these interactions.
The hybcell - and therefore the array itself - is constantly moved (rotated) through the sample. In this way, the sample is rapidly and homogeneously agitated towards the probes of the surface, resulting in improved reproducibility, drastic reduction in reaction times and flexible assay formats (e.g. a sequence of scans and heating and/or cooling steps).
The resulting robustness and reproducibility enables innovative assay formats for clinical diagnostics.
Cube Dx' proprietary compact sequencing combines a multiplex screening of several respiratory viruses - among those SARS-Cov-2 - and a follow-up mutation analysis.
For the screening based on RT-PCR the sample RNA is first transcribed into DNA and later amplified. Specific Taqman probes show an amplification of target DNA in different channels (fluorescence dyes).
Positive SARS-Cov-2 samples are examined for mutations in a second step. The amplified DNA is transferred into hybcell and the reactions starts with hybridization of the labelled, single stranded DNA to immobilized probes on the hybcell’s surface. In the case of perfect 3’‑ complementarity, the immobilized probes are extended as a result of polymerase activity. These extended probes remain associated even at high temperatures whilst non-extended duplexes are removed during a washing step. The aim of compact sequencing is the differentiation of single bases which is used for examining mutations. The array offers a broad analysis of mutations of currently 4x 20 mutations (SNPs). The device software compiles a comprehensive report and associates likely variants with the resulting mutation pattern.