Highly sensitive identification of bacterial and fungal DNA and resistance genes

Cube Dx molecular pathogen tests support microbiologists and infectious disease specialist in providing early indications of sepsis-causing pathogens in whole blood. As no microbiological culturing is necessary, time to result reduces tremendously.

 

This is particularly relevant for microorganisms that have been weakened by antimicrobial treatment or which demonstrate slow growth in or on microbiological nutrient media. The broad spectrum of the Cube Dx tests is ideally suited to test for pathogens causing sepsis and other severe infections. 

 

For such kind of testing a high number of negative results is expected. Therefore it must be ensured that negative results are due to negative samples and not caused by flaws in the process. Therefore we offer a stringent process control that undergoes the same procedures as the sample itself – including pathogen enrichment and DNA extraction.

 

Cube Dx' Internal Process Control (IPC) consists of frozen bacterial material that is dissolved within the sample. Both qPCR (during the amplification process) and probes on the hybcell (during the identification process) finally confirm the presence of the IPC.

 

Bacteria: 56 species + 11 genera

9 antibiotic resistance genes

Fungi: 19 species + 5 genera

Results for mixed infections

Versatile - for different sample types

Confirmation of negative results


PCR-Boxes: Bacteria (16S) / Fungi (28S) / Resistance

Detection of bacterial 16S / fungal 28S / resistance genes using (q)PCR device

12x 20µL mastermix

 

hybcell Bacteria / Fungi / Pathogens DNA 

Multiplex DNA-test of bacterial and fungal targets 

12x hybcell

 

IPC and PCR-Box IPC

Frozen solution of bacterial cells put into sample and PCR-mastermix for amplification of these cells

25x 20 µL IPC and 12x 20µL mastermix 


Available literature

Download
Fast and Cost-Effective Pathogen Diagnosis on an Automated DNA/Protein Diagnostics Platform Based on Cylindrical Microarrays (hybcells)
2014_09 KHWELS Fast and Cost-Effective P
Adobe Acrobat Dokument 340.7 KB
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Comparison of traditional blood culture and hyborg Dx in patients suspicious for sepsis
2016_04 KHFELDKIRCH ComparisonCultureSep
Adobe Acrobat Dokument 845.9 KB
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Sensitive Molecular Identification of Pathogens causing Implant and Tissue Infections (ITI)
2016_04 KHWELS ComparisonCultureTissue.p
Adobe Acrobat Dokument 136.3 KB