Highly sensitive identification of bacterial and fungal DNA and resistance genes

Cube Dx molecular pathogen tests support microbiologists and infectious disease specialist in providing early indications of sepsis-causing pathogens in whole blood. As no microbiological culturing is necessary, time to result reduces tremendously.


This is particularly relevant for microorganisms that have been weakened by antimicrobial treatment or which demonstrate slow growth in or on microbiological nutrient media. The broad spectrum of the Cube Dx tests is ideally suited to test for pathogens causing sepsis and other severe infections. 


For such kind of testing a high number of negative results is expected. Therefore it must be ensured that negative results are due to negative samples and not caused by flaws in the process. Therefore we offer a stringent process control that undergoes the same procedures as the sample itself – including pathogen enrichment and DNA extraction.


Cube Dx' Internal Process Control (IPC) consists of frozen bacterial material that is dissolved within the sample. Both qPCR (during the amplification process) and probes on the hybcell (during the identification process) finally confirm the presence of the IPC.


Your advantages:

  • Indication of 56 bacterial species / 11 genera and 11 resistance genes
  • Indication of 18 fungal species / 5 genera
  • Indication of mixed infection
  • Flexible testing (bacteria or fungi, bacteria and fungi)
  • Unit use reagents for steady cost per sample (independent of number of tested samples)
  • Versatile - use different sample types: whole blood, blood cultures, tissue...
  • Safeguarding negative results by comprehensive process control

PCR-Boxes: Bacteria (16S) / Fungi (28S) / Resistance

20 µL eluate, 80-90 min.

Detection of bacterial 16S / fungal 28S and resistance gene -DNA using (q)PCR devices


hybcell Bacteria / Fungi / Pathogens DNA xA/xB

16S/28S DNA, 20 min.

Multiplex DNA-Test of bacterial and fungal targets 



20 µL frozen solution of bacterial cells (into sample)

20 µL eluate (into PCR), 80-90 min. (same protocol)


Available literature

Fast and Cost-Effective Pathogen Diagnosis on an Automated DNA/Protein Diagnostics Platform Based on Cylindrical Microarrays (hybcells)
2014_09 KHWELS Fast and Cost-Effective P
Adobe Acrobat Document 340.7 KB
Comparison of traditional blood culture and hyborg Dx in patients suspicious for sepsis
2016_04 KHFELDKIRCH ComparisonCultureSep
Adobe Acrobat Document 845.9 KB
Sensitive Molecular Identification of Pathogens causing Implant and Tissue Infections (ITI)
2016_04 KHWELS ComparisonCultureTissue.p
Adobe Acrobat Document 136.3 KB