Sensitive identification of pathogens from human whole blood requires the enrichment of pathogen DNA and the removal of human DNA (from blood cells).
With that the conditions for follow-up PCR improve significantly. Furthermore the "background data" (= human DNA) for NGS based pathogen analysis is reduced.
GINA Pathogen Enrichment kits handle up to 0.5 mL of sample (whole blood) and use standard laboratory equipment as a centrifuge and a heating block. The kits can be combined with different commercial DNA purification kits (manual or automated kits).
Together with Cube Dx’ Pathogen Identification hybcells, life-threatening infections like sepsis can be determined after 3 hours – from whole blood samples.
LINA modulation buffers shorten the time of pathogen identification significantly as no DNA extraction process is necessary. The PCR mix is directly fed with the mixture of modulation buffer and sample. The whole process of the pathogen identification (PCR + hybcell) is the same and pathogens and resistence genes are presented in the report.
LINA modulation buffer is used for samples with a relatively high concentration of pathogens, for example positive blood cultures, BAL, urine or swabs.
Together with Cube Dx’ Pathogen Identification hybcells, pathogens and resistence genes can be tested with minimal hands on and in less than 2 hours from samples like positive blood cultures.