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Sepsis diagnostics: why every hour decides treatment success

In sepsis, the chance of survival falls with every hour until effective antimicrobial therapy. Molecular diagnostics straight from whole blood can create the decisive head start.

12. May 2026 · cube dx
Sepsis diagnostics: why every hour decides treatment success

Time is the most critical factor

Bloodstream infections and the resulting sepsis are among the most common causes of morbidity and mortality in hospitals. What decides the outcome is the early identification of the causative pathogen and its resistance profile — only then can antimicrobial therapy be targeted.

Blood culture remains the gold standard, but incubation and subculturing often mean one to several days to a result. That delay is especially critical in patients with hard-to-culture or slow-growing pathogens — or when antimicrobial therapy has already started.

Molecular diagnostics straight from whole blood

Molecular tests can substantially shorten the time to result. cube dx’s compact sequencing identifies clinically relevant bacteria, fungi and resistance genes directly from whole blood in under three hours — with no prior culturing.

This approach is particularly valuable for:

  • Mixed infections, where several pathogens must be detected in parallel
  • Slow- or non-growing microorganisms
  • Situations where antimicrobial therapy is already under way

A complement, not a replacement

Molecular tests don’t replace blood culture — they add speed to it. In independent clinical studies, the hybcell technology showed advantages over established methods particularly for mixed infections and the rapid detection of relevant resistance markers. See the details in our publications.

Publications

Publications & studies

33rd ECCMID, Copenhagen · 2023

Comparison of two molecular assays and MALDI-TOF MS Sepsityper for the rapid identification of pathogens from positive blood cultures

P. Starzengruber, N. Ankowitsch, B. Selitsch, B. Willinger, A. Makristathis

Independent evaluation on 410 positive blood cultures: hybcell Pathogens DNA reached a 94.9 % identification rate on-panel and detected all pathogens in 45.5 % of polymicrobial infections — versus 6.8 % for MALDI-TOF Sepsityper — plus resistance markers only detectable by PCR.

Division of Clinical Microbiology, Medical University of Vienna

33rd ECCMID, Copenhagen · 2023

Diagnostic value of blood-culture-independent molecular tests for the diagnosis of bloodstream infections in neutropenic patients with fever

P. Starzengruber, I. Camp, M. Kussmann, F. Ratzinger, W. R. Sperr, K. Gleixner, B. Selitsch, H. Burgmann, B. Willinger, A. Makristathis

Prospective study in febrile neutropenic patients directly from whole blood: of three commercial molecular tests, only hybcell Pathogens DNA showed acceptable performance (best overall ROC-AUC 0.77), with results available in as little as three hours.

Medical University of Vienna

33rd ECCMID, Copenhagen · 2023

Detection of bacterial pneumonia pathogens in patients with COVID-19 using a commercial microarray in comparison with a commercial multiplex PCR and culture

H. L. Verhasselt, M. I. Sanders, F. Herbstreit, S. Dubler, D. Schmidt, J. Buer, P.-M. Rath

100 respiratory samples (mostly BAL) from ICU COVID-19 patients: the hyborg RED2 / hybcell assay detected Haemophilus influenzae best of all methods (20 vs 3 vs 0) with a 2-hour time-to-result and minimal hands-on time.

Institute of Medical Microbiology, University Hospital Essen (University of Duisburg-Essen)