# Publications & Studies

> Independent clinical evaluations of hybcell technology and our products - conducted at various hospitals and medical universities and presented at (microbiology) conferences.


## Comparison of two molecular assays and MALDI-TOF MS Sepsityper for the rapid identification of pathogens from positive blood cultures
P. Starzengruber, N. Ankowitsch, B. Selitsch, B. Willinger, A. Makristathis — Division of Clinical Microbiology, Medical University of Vienna. 33rd ECCMID, Copenhagen, 2023.

Independent evaluation on 410 positive blood cultures: hybcell Pathogens DNA reached a 94.9 % identification rate on-panel and detected all pathogens in 45.5 % of polymicrobial infections — versus 6.8 % for MALDI-TOF Sepsityper — plus resistance markers only detectable by PCR.

[Poster (PDF)](https://www.cubedx.com/publications/eccmid2023-lina-vs-sepsityper.pdf)

## Diagnostic value of blood-culture-independent molecular tests for the diagnosis of bloodstream infections in neutropenic patients with fever
P. Starzengruber, I. Camp, M. Kussmann, F. Ratzinger, W. R. Sperr, K. Gleixner, B. Selitsch, H. Burgmann, B. Willinger, A. Makristathis — Medical University of Vienna. 33rd ECCMID, Copenhagen, 2023.

Prospective study in febrile neutropenic patients directly from whole blood: of three commercial molecular tests, only hybcell Pathogens DNA showed acceptable performance (best overall ROC-AUC 0.77), with results available in as little as three hours.

[Poster (PDF)](https://www.cubedx.com/publications/eccmid2023-neutropenic-fever.pdf)

## Detection of bacterial pneumonia pathogens in patients with COVID-19 using a commercial microarray in comparison with a commercial multiplex PCR and culture
H. L. Verhasselt, M. I. Sanders, F. Herbstreit, S. Dubler, D. Schmidt, J. Buer, P.-M. Rath — Institute of Medical Microbiology, University Hospital Essen (University of Duisburg-Essen). 33rd ECCMID, Copenhagen, 2023.

100 respiratory samples (mostly BAL) from ICU COVID-19 patients: the hyborg RED2 / hybcell assay detected Haemophilus influenzae best of all methods (20 vs 3 vs 0) with a 2-hour time-to-result and minimal hands-on time.

[Poster (PDF)](https://www.cubedx.com/publications/eccmid2023-lina-bal.pdf)

## Molecular Microbiology — literature
The technology has been evaluated in independent studies. A selection of available literature:

- 2016: [Sensitive Molecular Identification of Pathogens causing Implant and Tissue Infections (ITI)](https://www.cubedx.com/literature/2016KHWELSComparisonCultureTissue.pdf)
- 2020: [Molecular pathogen identification and resistance gene detection from positive blood cultures](https://www.cubedx.com/literature/2020ECCMIDAbstract-LINApositiveBC.pdf)
- 2020: [Presence and distribution of fungal species and dermatophytes in nail and skin samples](https://www.cubedx.com/literature/2020ECCMIDAbstract-Dermatophytes.pdf)
- 2021: [SMARTDIAGNOS – next generation molecular sepsis diagnosis technology for whole blood samples](https://www.cubedx.com/literature/2021ECCMIDPoster-SMARTDIAGNOSSepsis.pdf)
- 2022: [Extraction-free Molecular Identification of Bacteria/Fungi Directly from BAL Samples](https://www.cubedx.com/literature/2022CDXAppNoteLINABAL.pdf)
- 2023: [Comparison of two molecular assays and MALDI-TOF MS Sepsityper for the rapid identification of pathogens from positive blood cultures](https://www.cubedx.com/literature/2023ECCMIDPoster-LINAvsSepsityperMUV.pdf)
- 2023: [Detection of bacterial pneumonia pathogens in patients with COVID-19](https://www.cubedx.com/literature/2023ECCMIDPoster-LINAwithBALMUE.pdf)
- 2023: [Diagnostic value of blood culture independent molecular tests for the diagnosis of bloodstream infections in neutropenic patients with fever](https://www.cubedx.com/literature/2023ECCMIDPoster-NeutropfeverComparisonbloodtestMUV.pdf)
- 2026: [Performance comparison to in-house PCR and 16S sequencing](https://www.cubedx.com/literature/ESCMID2026POSTER-ComparisonPCR16SHAUKELANDUNIVERSITY.pdf)
- 2026: [Infective endocarditis: an alternative molecular diagnostic approach to improve laboratory analysis of heart valve tissue](https://www.cubedx.com/literature/ESCMID2026POSTER-EndocarditsHOSPITALVERONA.pdf)
- 2026: [Rapid molecular detection directly from whole blood for the early diagnosis of paediatric bloodstream infections using the cube dx system](https://www.cubedx.com/literature/ESCMID2026POSTER-BSITestingChildrenBAMBINOGESU.pdf)