16S & 28S amplicon sequencing

GINA and PCR-Box also work outside the hybcell workflow: as sample preparation and PCR for your own 16S and 28S amplicon sequencing — Sanger or NGS.

Why it matters

Early results - optimised therapy

Earlier to result

Comprehensive pathogen ID directly from whole blood and other sample matrices in less than 3 hours — no culturing required.

Additional results

Additional results from DNA-based detection of pathogens that are difficult to culture (fungi, fastidious bacteria, anaerobes) or for patients who have already undergone treatment.

Versatile multiplexing

Sepsis, other severe infections (pneumonia, etc.), or general 16S/28S amplicon sequencing—the same platform, the same products, multiple applications.

Amplicon sequencing · 16S / 28S, Sanger or NGS

16Span-bacterial PCR · plus 28S pan-fungal

Sample prep: Enrichment of microbes; DNA extraction; Columns or magnetic beads

PCR-Box Bacteria is a pan-bacterial 16S PCR mix, PCR-Box Fungi a pan-fungal 28S PCR mix. Beside compact sequencing on the hybcell, the amplicons are ready for state-of-the-art 16S and 28S sequencing (Sanger, NGS). From whole blood, GINA first depletes human DNA and enriches the microbial DNA.

Ready-to-use PCR mixes, single reactions in 0.2 mL tubes
Extremely sensitive, with the possibility of RNA transcription
Hot-start functionality and UNG carry-over protection
Manufactured under cleanroom conditions

01
Sample preparation
- GINA 500
02
DNA extraction & PCR
- GINA DNA Purification
- 3rd party DNA extraction
- PCR-Box Bacteria
- PCR-Box Fungi
03
Sequencing
- Sanger
- NGS