Sepsis and other severe infections are mainly caused by bacteria or fungi. Early identification of the causative pathogen is crucial for effective and successful treatment with antibiotics, antimycotics, supporting medication and other treatments.
Cube Dx molecular pathogen tests support microbiologists and infectious disease clinicians in providing very early indication of a pathogen in whole blood and other sample types. This is particularly relevant for microorganisms that have been weakened by antimicrobial treatment or which demonstrate slow growth in or on microbiological nutrient media. The multiplex technology employed in Cube Dx is ideally suited to allow for identification in parallel of a wide spectrum of possible pathogens.
A small amount of genomic DNA from a selection of popular DNA extraction kits is used as a sample.
This DNA is added to a (q)PCR vessel together with a reaction mixture and amplified by a (q)PCR reaction. For the detection of bacteria and the detection of fungi, 16S- and 28S-rDNA respectively are used. For the detection of resistance markers, different gene fragments are amplified. The reaction is designed so that the resulting DNA strands undergo labelling with a fluorescent marker.
When using a qPCR device, negative samples can be singled out after the PCR reaction and not processed any further. The positive samples are further processed and subjected to the identification step.
The amplified material is pipetted into the hybcell cartridge which is then inserted into the hyborg – a fully-automated device. Multiple samples can be prepared and transferred in the same way and at the same time.
The amplified DNA binds to immobilised probes on the hybcell surface. In the case of a perfect match, these primers are extended by a highly specific DNA polymerase. Subsequently, the amplified and labelled material remains bound to the lengthened primers, even at higher temperatures. Any amplified material which has not been extended along the bound probe is removed by a stringent washing step.
The measured values are interpreted by the software and displayed in tabular form for each microorganism (positive / negative).
- Test for both gram-negative and gram-positive bacteria
- Test for fungi
- Test for antibiotic resistances
The test portfolio is constantly being extended to correspond with the development of sepsis therapy.
hybcell Fungi DNA xA for Sepsis Testing - English
on Cylindrical Microarrays (hybcells) - English
Implant and Tissue Infections (ITI)- English